Project

Part:BBa_K3218002:Design

Designed by: Abraham Grosman   Group: iGEM19_MSP-Maastricht   (2019-10-15)


a 'dead' Cas9; dCas9 is an inactive endonucelase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3432
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 977
    Illegal BglII site found at 1436
    Illegal BglII site found at 1525
    Illegal BglII site found at 2170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design had to be tweaked depending on the endonuclease that was to be fused. Therefore for the complexes formed in this project the sequences differ slightly to accommodate these differences.


Source

Cas9 is part of a defense mechanism in some bacteria to prevent the integration of viral DNA into their genome.

References